This A-Level Biology paper investigates the fundamental principles of enzyme activity, focusing on the biochemical mechanisms that drive enzyme-catalyzed reactions. It explores the roles of enzyme structure, active sites, and substrate specificity in facilitating biological processes. Key factors affecting enzyme activity, including temperature, pH, and substrate concentration, are discussed with reference to experimental data. The paper also looks at enzyme inhibition and its relevance to metabolic regulation and pharmaceutical development. Real-world applications, such as the use of enzymes in industrial processes and biotechnology, are considered to highlight the importance of enzymes in both biology and industry.

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